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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cell Signal. 2009 May 18;21(9):1423–1435. doi: 10.1016/j.cellsig.2009.05.006

Fig. 3. Dominant-negative forms of Src and FAK do not block BCAR3-mediated p130Cas phosphorylation.

Fig. 3

Panel A: Co-transfection with Src and FAK results in accumulation of more slowly migrating p130Cas species, although the level of p130Cas tyrosine phosphorylation observed is far greater following transfection with Src than with BCAR3. MCF-7 cells were transiently transfected with HA-tagged p130Cas (HA-Cas) in combination with wildtype Fak, Src or HA-BCAR3, followed by Western blotting for anti-phosphotyrosine (anti-P-Y), HA, Src or FAK, as indicated. Panel B: Co-expression of dominant negative Src (DN-Src) does not alter the BCAR3-mediated reduction in p130Cas migration. MCF-7 cells were transiently transfected with HA-Cas, HA-BCAR3, and dominant-negative Src (DN-Src). Either one-half (0.5) or one-quarter (0.25) as much DN-Src plasmid was transfected as HA-Cas and HA-BCAR3. Panel C: Co-expression of dominant negative FAK (DN-FAK) does not alter the BCAR3-mediated reduction in p130Cas migration. MCF-7 cells were transfected as in Panel B but with DN-FAK rather than DN-Src.