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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Biochem Pharmacol. 2007 Aug 19;74(11):1665–1676. doi: 10.1016/j.bcp.2007.08.014

Fig. 3. Regulatory mechanism characterization of female-predominant Ntcp expression in mouse liver.

Fig. 3

The solid, black bar represents Ntcp mRNA in males; the striated bar depicts Ntcp mRNA in females. Total liver RNA was isolated and analyzed by the bDNA signal amplification assay for Ntcp mRNA content. The data are presented as mean ± SEM (each group, n = 5 mice). a) Effects of gonadectomy and sex hormone replacements on mouse Ntcp mRNA expression in livers from intact and gonadectomized male and female mice. GNX, placebo administered to gonadectomized mice; GNX + DHT, 5α-dihydroxytestosterone administered to gonadectomized mice; and GNX + E2, 17β-estradiol administered to gonadectomized mice. Asterisks (*) represent statistically significant differences (p < 0.05) between males and females in intact mice and the same treated gonadectomized mouse group; single dagger (†) represent statistically significant differences (p < 0.05) between intact mice and the same gender, untreated gonadectomized mice; and double dagger (‡) represents statistically significant differences (p < 0.05) between untreated gonadectomized mice and the same gender, gonadectomized mice administered 5α-dihydroxytestosterone, or 17β-estradiol. b) Effects of hypophysectomy and growth hormone on mouse Ntcp mRNA expression in livers from intact and hypophysectomized male and female mice. HX, placebo administered to hypophysectomized mice; HX+GHM, rat growth hormone twice daily administered by intraperitoneal injection to hypophysectomized mice mimicking male-pattern growth hormone secretion; and HX+GHF, continuous infusion to hypophysectomized mice via subcutaneously implanted 21-day-release 1mg rat GH pellet mimicking female-pattern growth hormone secretion. Asterisks (*) represents statistically significant differences (p < 0.05) between males and females in intact mice and the same treated hypophysectomized mouse group; single dagger (†) represent statistically significant differences (p < 0.05) between intact mice and the same gender, untreated hypophysectomized mice; and double dagger (‡) represents statistically significant differences (p<0.05) between untreated hypophysectomized mice and the same gender, or hypophysectomized mice following hormone replacement treatments. c) Effects of growth hormone on mouse Ntcp mRNA expression in livers from intact and lit/lit male and female mice. Lit/lit, placebo administered to Lit/lit mice; Lit/lit +GHM, rat growth hormone twice daily administered by intraperitoneal injection to Lit/lit mice mimicking male-pattern growth hormone secretion; Lit/lit+GHF, continuous infusion to Lit/lit mice via subcutaneously implanted 21-day-release 1mg rat growth hormone pellet mimicking female-pattern growth hormone secretion.