Table 1. Oligonucleotide probes generated for analysis of mouse Ntcp and Bsep mRNAs expression by Quantigene branched DNA signal amplification assay.
| Ntcp (MMU95131a) | Bsep (NM_021022) | ||
|---|---|---|---|
| CEb | gccttgatcttgctgaactccaTTTTTctcttggaaagaaagt | CE | ggtcgagaaggatatgtaaatttacaTTTTTctcttggaaagaaagt |
| CE | gaggatggccagagcctcaTTTTTctcttggaaagaaagt | CE | atgtttgaacggaggaactgaaTTTTTctcttggaaagaaagt |
| CE | ttgctgtagatgtataagaggagaggTTTTTctcttggaaagaaagt | CE | tttgcggcagctatagctctcTTTTTctcttggaaagaaagt |
| CE | gccttttggacttcaggaagatcTTTTTctcttggaaagaaagt | CE | ccgcgagagagttgagagccTTTTTctcttggaaagaaagt |
| LE | tgtctgtggcccggtggTTTTTaggcataggacccgtgtct | CE | ccctctctggctttgtccagagTTTTTctcttggaaagaaagt |
| LE | agaatgacgctgagcgcagTTTTTaggcataggacccgtgtct | LE | tgcccaggatccacagataccTTTTTaggcataggacccgtgtct |
| LE | catgatgagcagcaacataactaccTTTTTaggcataggacccgtgtct | LE | ccaacgaatgccagcgtcTTTTTaggcataggacccgtgtct |
| LE | tggtgcagccaagcgagagTTTTTaggcataggacccgtgtct | LE | gcttttaccacacccactgctcTTTTTaggcataggacccgtgtct |
| LE | gccactatggcgatgatcaccTTTTTaggcataggacccgtgtct | LE | tccaacagctggatgctggtTTTTTaggcataggacccgtgtct |
| LE | caggagagcagccgcagatTTTTTaggcataggacccgtgtct | LE | tgatcgggatcgtagaaccgtTTTTTaggcataggacccgtgtct |
| LE | aagaggttagacaggttcccccTTTTTaggcataggacccgtgtct | LE | ccatctatcatcaccgttcccTTTTTaggcataggacccgtgtct |
| LE | cacaatgctgaggttcatgtccTTTTTaggcataggacccgtgtct | LE | cattgacttttttgctgtcgtgaTTTTTaggcataggacccgtgtct |
| LE | aagctggagcaggtggtcatTTTTTaggcataggacccgtgtct | LE | gctcctgggagacaatcccaTTTTTaggcataggacccgtgtct |
| LE | ttaagatctccgtcgtagattcctTTTTTaggcataggacccgtgtct | LE | cattatgctacagtcaaataacacggTTTTTaggcataggacccgtgtct |
| LE | cctttgtagggcaccttgtccTTTTTaggcataggacccgtgtct | LE | ttgtccccatacttgatgttgtcTTTTTaggcataggacccgtgtct |
| LE | cctatggcgcaaggaatgaTTTTTaggcataggacccgtgtct | LE | agtcatgcagctgagcctgcTTTTTaggcataggacccgtgtct |
| BL | cctttgggcttccagaagtga | LE | ctggatcccaacattagtttcatatTTTTTaggcataggacccgtgtct |
| BL | ggggcatgataccgtactgg | LE | aatagcaatgcgttgtttctccTTTTTaggcataggacccgtgtct |
| BL | gcccagaaggaaagcactga | LE | tcgtacaatggcccgagcTTTTTaggcataggacccgtgtct |
| BL | atgctggtcagatgaaagacctt | LE | catccagtagcaagattttaggatcTTTTTaggcataggacccgtgtct |
| BL | cccttcatggccagggtg | LE | tctgtgtctaaggcagatgtagcttTTTTTaggcataggacccgtgtct |
| BL | catcatgcccaaggcagtg | LE | caagctgcactgtcttttcacttTTTTTaggcataggacccgtgtct |
| BL | gaaccatgacgagtgataacataatg | LE | agcaatgacaatacaggtccgaTTTTTaggcataggacccgtgtct |
| LE | agttctggatagtagacaagcgatgTTTTTaggcataggacccgtgtct | ||
| LE | gacatgacggcgatgatatctgTTTTTaggcataggacccgtgtct | ||
| BL | gacagtccattcagaacttgtatatca | ||
| BL | tccacggagatctctttggtg | ||
| BL | ttctctgggagtgacatgacga | ||
a
GenBank accession numbers for each transcript are given in parenthesis after the gene name.
b
The type of Function of each bDNA oligonucleotide probe. CE, capture extender; LE, label extender; BL, blocker.