(A) Day-7 and Day-21 embryoid bodies (EB7 and EB21) derived from LYON-ES1cells. (B) RT-PCR analysis of ES cell- and lineage- markers expression in LYON-ES1 cells, spontaneously differentiated cells (DIF), EB7 and EB21. (C) Teratomas sections six weeks after injection of LYON-ES1 cells in the testes of SCID mice, showing derivatives of the ectoderm, endoderm and mesoderm. Scale bars= 500μm (A, C). Abbreviations: Mefs, mouse embryonic fibroblasts; W, water control; Bra, Brachyury; K, keratin.