Figure 4.

The influences of diabetes on cytoplasmic and membranous GLUT4 protein level were examined in the age-matched controlled, STZ-diabetic, and insulin-treated diabetic rats with or without I/R injury. Equal amounts of proteins were resolved on 10% SDS-PAGE and blotted with respective GLUT4 antibodies. All experiments were performed in triplicates from six animals (*, vs. CS; †, vs. DMS; ‡, vs. C; n = 6 per group). Upper panels show blots, and quantified ratio is shown in the lower panels. C, age-matched control; CS, control sham; DM, STZ-induced diabetes; DMS, DM sham; DMI, diabetes treated with insulin (4 IU/day for 5 days); DMIS, DMI sham; I/R, myocardial ischemia 1 h following by a 3 h of reperfusion.