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. 2009 Sep 18;4(9):e7064. doi: 10.1371/journal.pone.0007064

Figure 3. Manipulation of cyclin B1 levels.

Figure 3

Lysates were made from known numbers of PC3 and DU145 cells that had either been grown to confluence or harvested at very low density. Equal numbers of cells were loaded in each lane and Western analysis of cyclin B1 was performed. The increase in total cyclin B1 in subconfluent relative to confluent cells was 4.4 fold for PC3 cells and 2.5 fold for DU145 cells (A). Nocodazole (NDZ) treatment for the indicated hours produced a final 17.6 fold increase over confluent cells for DU145 cells (B) and a 52 fold increase for PC3 cells (C).