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. 2009 Sep 18;4(9):e7064. doi: 10.1371/journal.pone.0007064

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Frisa, Jacobberger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The cytometric data for HeLa, K562, and RKO cells (Figure 7) were gated to exclude endoreduplicated or binucleate cycling cells (4C 8C); mitotic cells after metaphase, and outliers. The purpose was to expose only the cells distributed about the two dimensional center as a function of DNA content. These data represent the most common cycling cell in the population. Arbitrary regions were set, moving through the data from early G1 through late G2+M (A–C). The mean cyclin B1 levels from each region are plotted as a function of the cell frequency in the region (D–F). The frequency data were renormalized from the first non-zero cyclin B1 point and plotted to compare the committed part of the cell cycle (cyclin B1 is detectable) for each cell line (G). In A-G, the X axis scale is 0–1, and the Y axis scale is 0–4×10⁶.

Inline graphic — The cytometric data for HeLa, K562, and RKO cells (Figure 7) were gated to exclude endoreduplicated or binucleate cycling cells (4C 8C); mitotic cells after metaphase, and outliers. The purpose was to expose only the cells distributed about the two dimensional center as a function of DNA content. These data represent the most common cycling cell in the population. Arbitrary regions were set, moving through the data from early G1 through late G2+M (A–C). The mean cyclin B1 levels from each region are plotted as a function of the cell frequency in the region (D–F). The frequency data were renormalized from the first non-zero cyclin B1 point and plotted to compare the committed part of the cell cycle (cyclin B1 is detectable) for each cell line (G). In A-G, the X axis scale is 0–1, and the Y axis scale is 0–4×10⁶.