Figure 1 Expression studies during palate fusion in mouse. (A) RNA in situ hybridisation of the Myh9 and transforming growth factorβ3 (Tgfβ3) genes in adjacent sections of embryonic mouse at day 14.5 showing an overlapping pattern of expression. (B) Immunohistochemistry using antibodies against heavy chain of non‐muscle myosin IIA (NMMHCIIA) during palate fusion on mouse sections at embryo day 14.5. The arrows indicate the medial epithelial seam (MES) with the highest expression levels of Myh9 mRNA and protein. n, nasal cavity; ns, nasal septum; o, oral cavity; ps, palate shelf; t, tongue. Scale bars represent 100 μm.