Table 1 Mutations found in microdissected tumour samples.
| Slides | Tissue cell types | CTNNB1 mutation | Nucleotide change | WT1 mutation* | LOH analysis |
|---|---|---|---|---|---|
| Initial left tumour | |||||
| A3 | Tubuli | S45P | TCT>CCT | m/m | |
| A3 | Stroma | T41A | ACC>GCC | m/m | |
| A3 | Blastema | S45P | TCT>CCT | m/m | |
| A3 | Stroma II | S45P | TCT>CCT | nd | |
| A3 | Muscle I | S45P | TCT>CCT | m/m | |
| A3 | Muscle II | S45P | TCT>CCT | nd | |
| A1 | Muscle | S45P | TCT>CCT | nd | |
| A1 | Stroma | S45P | TCT>CCT | nd | |
| A1 | Muscle/fat | S45P | TCT>CCT | nd | |
| Initial right tumour | |||||
| C2 | Tumour pool | ΔS45 | ΔTCT | nd | |
| C2 | Muscle I | ΔS45 | ΔTCT | m/m | |
| C2 | Muscle II | ΔS45 | ΔTCT | nd | |
| C2 | Normal kidney I | wild type | m/w | ||
| C2 | Normal kidney II | wild type | nd | ||
| Recurrence left | |||||
| 3B | Muscle I | S45F | TCT>TTT | nd | |
| 3B | Muscle II | S45F | TCT>TTT | nd | |
| Cell line | S45F | TCT>TTT | m/m | LOH 11p13 + p15 no LOH 11q11 | |
| Recurrence right | |||||
| 1E | Blastema I | S45C | TCT>TGT | nd | LOH 11p13 no LOH 11q11 |
| 1E | Blastema II | S45C | TCT>TGT | nd | |
| 1E | Muscle | S45C | TCT>TGT | nd | |
| 1E | Stroma | S45C | TCT>TGT | nd | |
| F1 | Blastema I | S45C | TCT>TGT | nd | LOH 11p15 |
| F1 | Blastema II | S45C | TCT>TGT | nd | LOH 11p15 |
| F1 | Epithelia I | wild type | nd | no LOH 11p15 | |
| F1 | Epithelia II | wild type | nd | no LOH 11p15 | |
| F1 | Muscle | S45C | TCT>TGT | nd | LOH 11p15 |
Microdissected areas labelled in italics correspond to normal kidney cells by molecular analysis, one WT1 mutant allele as in all cells of the patient and no CTNNB1 mutation.
*WT1 mutation analysis was performed by sequencing, m/m indicates mutant/mutant allele, and m/w one mutant one wild type allele, nd: not done