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A, Polymerase chain reaction (PCR) detection of Trypanosoma cruzi DNA in cardiac tissue using primers TCZ1 and TCZ2. Lane 1: control animal; Lanes 2–5: infected animals; Lanes 6–7: positive controls (T. cruzi DNA, Tulahuen strain); Lane 8: distilled water; lane MK: molecular weight markers. B, PCR detection of T. cruzi DNA in spleen using primers TCZ1 and TCZ2. Lanes 1–5: uninfected control animal; Lanes 6–8: infected animal; Lane 9: distilled water; lane MK: molecular weight markers.
