Table 3.

Primers and probes used in real-time polymerase chain reaction (PCR) for quantification of Trypanosoma cruzi DNA

T. cruzi	Forward	5′-TGC ACT CGG CTG ATC GTT T-3′
	Reverse	5′-CAA AAA TTT AGA CCA CAA CGT GTG A-3′
	TaqMan probe	6FAM-CGA GCG GCT GCT G-MGBNFQ
Peptidylprolyl isomerase A (Cyclophilin A)	Forward	5′-GCA AAG TGA AAG AAG GCA TGA A-3′
	Reverse	5′-CCA TTC CTG GAC CCA AAG C-3′
	TaqMan probe	6FAM-TCC ATG GCC TCC ACA AT-MGBNFQ