Skip to main content
. Author manuscript; available in PMC: 2009 Sep 10.
Published in final edited form as: Mol Cancer Res. 2008 Oct;6(10):1521–1533. doi: 10.1158/1541-7786.MCR-07-2203

FIGURE 3.

FIGURE 3

Conditioned medium from Tgfbr2FspKO fibroblasts enhances HGF signaling in mammary carcinoma cells. A. PyVmT (left panel) or 4T1 (right panel) carcinoma cells were treated with basal medium containing DMEM/F12/0.5% ABS, HGF (60 ng/ml) or fibroblast conditioned medium from Tgfbr2Flox/Flox control or Tgfbr2FspKO fibroblasts, and analyzed by western blot analysis for phosphorylation status of indicated proteins. Experiments were performed in quaduplicate with duplicate samples. Representative results are shown. B. Northern blot analysis of Tgfbr2Flox/Flox control and Tgfbr2FspKO fibroblasts. C. 4T1 cells were treated with fibroblast conditioned medium in the presence or absence of anti-HGF or control IgG and analyzed by western blot for the phosphorylation status of indicated proteins.