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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Virology. 2009 Jul 23;392(1):16–23. doi: 10.1016/j.virol.2009.06.035

Figure 3. Intact C/EBPα sites are required for amplification of oriLyt.

Figure 3

(A) Schematic of oriLyt sequence from nucleotides 92,174-92,541 showing the positions of C/EBPα transcription factor binding sites and the mutations made within the oriLyt sequence. (B) Southern blot of a transient transfection replication assay. HF cells were transfected with various oriLyt containing plasmids plus the internal control plasmid pALTER-OriLyt. Cells were infected 24 h post transfection and total cell DNA was harvested 5 days post transfection and cleaved with DpnI and EcoRI. Following agarose gel separation and transfer to a nylon membrane, the blot was hybridized to a 32P-labbled-pGEM probe. Arrows to the left of the figure show the identification of each plasmid.