Fig. 2.
β3AR-induced IL-6 expression is PKA- and Epac-independent. C3H10T1/2 cells were pretreated with H89 (10 μM for 1 h) prior to stimulation with CL316243 (1 μM for 2 h). The CL316243-induced activation of the CRE reporter was not inhibited by H89 in the homogenous reporter assay (a). 3T3-L1 cells were treated with KT5720 (10 μM) or H89 (5-10 μM) for 1 h prior to treatment with CL316243 (1 μM for 45 min). The CL316243-induced up-regulation of IL-6 mRNA was not inhibited by KT5720 or H89 (b). Treatment of 3T3-L1 with CTX (100 ng/ml for 4 h) led to increases in IL-6 transcript, while treatment with the Epac agonist 8CPT-2’-O-Me-cAMP (CPTOMe) (10 μM for 45 min) did not enhance IL-6 expression (c). ***P<0.001 and **P<0.01 different from untreated.