| Solution A | Solution B |
| 0.6% NP-40 | 25% glycerol |
| 150mM NaCl | 20mM Hepes (pH 7.9) |
| 10mM Hepes (pH 7.9) | 420mM NaCL |
| 1mM EDTA | 1.2mM MgCl |
| 0.5mM PMSF (add Fresh!) | 0.2mM EDTA |
| 0.5mM DTT (add Fresh) | |
| 0.5mM PMSF (add Fresh) | |
| protein inhibitor cocktail (add fresh according to manufacturer) |
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