Table 1.
Information pertaining to primary antibodies
Antibody | Source | Concentration | Epitope | References |
---|---|---|---|---|
Anti-acetylated tubulin | SIGMA | 1 : 1000 | Acetylated tubulin | Svoboda et al. (2001) |
Anti-engrailed (4D9) | DSHB* | 1 : 200 | Engrailed, muscle pioneers | Patel et al. (1989), Ekker et al. (1992) |
zn1 | DSHB* | 1 : 200 | Neuronal marker (cytoplasmic) | Trevarrow et al. (1990) |
znp1 | DSHB* | 1 : 250 | Motoneuron axons | Trevarrow et al. (1990) |
zn5 | DSHB*,† | 1 : 1000 | DM-GRASP, secondarymotoneuron axons | Fashena & Westerfield (1999) |
zn12 | DSHB* | 1 : 250 | HNK-1, neuronal axons | Metcalfe et al. (1990) |
F59 | DSHB* | 1 : 50 | Myosin heavy chain, adaxial(slow) muscle cells | Crow & Stockdale (1986),Miller et al. (1989) |
F310 | DSHB* | 1 : 250 | Myosin light chain, fastmuscle cells | Crow & Stockdale (1986) |
Developmental Studies Hybridoma Bank.
Available as zn8 from the Developmental Studies Hybridoma Bank and the Zebrafish International Resource Center.