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. Author manuscript; available in PMC: 2009 Sep 10.
Published in final edited form as: J Invest Dermatol. 2009 Jun;129(6):1483–1488. doi: 10.1038/jid.2008.374

Figure 2.

Figure 2

Acquisition of mutations in the VGP as determined by sequencing chromatograms. (A) Predetermined amounts of BRAF T1799A mutated DNA were mixed with wild type DNA. The contribution of the mutated DNA to the chromatogram reflects the percentage in the DNA mixture. The red peak (T) is wild type; the green peak (A) represents the single nucleotide mutation. (B) Three representative cases of DNA isolated from paired RGP/VGP samples, amplified with primers for BRAF exon 15, and sequenced. Measurement of the chromatograms reveals an increased percentage of T1799A mutations in the VGP DNA.