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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Biochemistry. 2009 Aug 4;48(30):7160–7168. doi: 10.1021/bi900830g

Figure 2.

Figure 2

Chemical shift changes caused by mutation. 1H-15N HSQC spectra for (A) wild-type, (B) RNase AECP, and (C) H48A enzymes. The spectra were acquired at 14.1 T and 298K, pH = 6.4. In (D) chemical shift differences for WT vs. H48A (blue) and WT vs. RNase AECP are shown as a function of amino acid sequence. Gray boxed areas depict protein regions with larger than average chemical shift disturbances. (Δ (ppm) = [(Δδ2HN + Δδ2 N/25)/2]1/2) in which δ is the difference in chemical shift between the two proteins for NH and HN nuclei (52).