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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Biochemistry. 2009 Aug 4;48(30):7160–7168. doi: 10.1021/bi900830g

Table 1.

3′-CMP binding constants for WT RNase A, RNase AECP and mutant H48A.

Enzyme Kd (μM) koff (s−1) kon (M−1s−1)a
WT Rnase A 74 ± 2 1155 ± 120 1.56 × 107 ± 1.67 × 106
RNase AECP 300 ± 16 96 ± 9 3.20 × 105 ± 3.45 × 104
RNase A (H48A) 895 ± 50 142 ± 19 1.59 × 105 ± 2.30 × 104
a

kon determined from koff/Kd.