Enzyme assays contained 0.5 mM coniferyl acetate, 1 mM NADPH, and 1 μg purified enzymes, and were incubated for 30 min at RT. Reaction products were analyzed by GC-MS. Relative abundance is shown in arbitrary units, based on Total Ion Current measurement.
(a) Reaction catalyzed by C. breweri IGS1.
(b) Reaction catalyzed by C. breweri EGS1.
(c) Reaction catalyzed by C. breweri EGS2.
(d) Reaction catalyzed by petunia EGS1.