Table 2.
Purification of IGS and EGS from C. breweri petals
| Purification Step | Total Protein (mg) |
Total Activity (pkat) |
Specific Activity (pkat • mg-1) |
Purification (-fold) |
Recovery (%) |
||||
|---|---|---|---|---|---|---|---|---|---|
| IGS | EGS | IGS | EGS | IGS | EGS | IGS | EGS | ||
| Crude Extract | 344.0 | 1448.1 | 367.5 | 4.2 | 1.1 | 1.0 | 1.0 | 100.0 | 100.0 |
| DE-53 | 98.6 | 671.4 | 330.3 | 6.8 | 3.4 | 1.6 | 3.1 | 46.4 | 89.9 |
| Hitrap-Phenyl (Peak 1)1 | 18.8 | 110.5 | 110.4 | 5.9 | 5.9 | 1.4 | 5.4 | 7.6 | 30.0 |
| Hitrap-Phenyl (Peak 2)1 | 3.1 | — | 158.0 | — | 50.6 | — | 46.0 | — | 43.0 |
| Hitrap-Q #1 | 3.6 | 139.6 | 107.4 | 38.8 | 29.8 | 9.2 | 27.1 | 9.6 | 29.2 |
| Hitrap-Q #2 | 0.9 | — | 108.1 | — | 118.8 | — | 108.0 | — | 29.4 |
1
There were two peaks of EGS activities eluting from the Hitrap-Phenyl column, and the single peak of IGS activity coincided with the first peak of EGS activity on this column, as described in the text. Data in this table labeled Peak 1 and Peak 2 were obtained from pooled fractions constituting each peak.