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Kinetic parameters of C. breweri and P. hybrida EGS and IGS enzymes for coniferyl acetate
| Enzymes |
Km (μM) |
Vmax (nmol • s-1 • mg-1) |
kcat (s-1) |
kcat/Km (s-1 • mM-1) |
|---|---|---|---|---|
| CbIGS1 | 211.5 ± 27.9 | 27.6 ± 3.7 | 0.99 ± 0.13 | 4.7 |
| CbEGS1 | 93.3 ± 6.3 | 7.3 ± 0.2 | 0.26 ± 0.01 | 2.8 |
| CbEGS2 | 310.5 ± 45.2 | 6.9 ± 0.6 | 0.25 ± 0.02 | 0.8 |
| PhEGS1 | 245.3 ± 58.0 | 18.4 ± 1.6 | 0.60 ± 0.05 | 2.4 |
| PhIGS1 | 226.1 ± 70.3 | 35.7 ± 5.9 | 1.3 ± 0.2 | 5.7 |
Values are averages of three independent experiments, +/- SE.
