Table 4.
Species | Mutant No. | Mutation | Product proportions (EGS : IGS) |
---|---|---|---|
Clarkia | CbIGS1 | Wild type | 0:100 |
1* | R73K, V77I, S83P, V84F, Y87I, P88S, L91I, L92H, D95N | 76:24 | |
2 | R73K, V77I, S83P, V84F | 29:71 | |
3 | S83P, V84F, Y87I, P88S | 85:15 | |
4 | L91I, L92H, D95N | 0:100 | |
5 | S83P, V84F | 16:84 | |
6 | Y87I, P88S | 66:34 | |
7 | V84F, Y87I | 75:25 | |
8 | V84F | 39:61 | |
9 | Y87I | 62:38 | |
CbEGS1 | Wild type | 100:0 | |
10 | F84V, I87Y | 31:69 | |
CbEGS2 | Wild type | 100:0 | |
11 | F86V, I89Y | 88:12 | |
Basil | ObEGS1 | Wild type | 100:0 |
12 | F85V, I88Y | 64:36 | |
Petunia | PhIGS1 | Wild type | 0:100 |
13 | V88F, Y91I | 39:61 | |
PhEGS1 | Wild type | 100:0 | |
14 | Q86V, L89Y | 81:19 |
Asterisk indicates the hybrid protein, CbEGS1/CbIGS1