Table 1.
Antigen | Clone | Dilution | HIER | Source |
---|---|---|---|---|
CD20 | L26 | 1:1200 | MW CB | Dako |
CD3 | SP7 | 1:200 | S CB | Lab Vision |
CD10 | 56C6 | 1:100 | S CB | Novocastra |
CD138 | B-B4 | 1:800 | PC CB | Invitrogen |
BCL-2 | 124 | 1:400 | MW CB | Dako |
BCL-6 | PG-B6P | 1:100 | T+ S TRIS | Dako |
MUM-1/IRF4 | MUM1P | 1:1200 | S CB | Dako |
Ki-67 | MIB-1 | 1:4800 | PC CB | Dako |
TdT | Polyclonal | 1:1600 | PC EDTA | Dako |
Dako, Carpinteria, CA, USA; Lab Vision Corporation, Fremont, CA, USA; Novocastra, United Kingdom; Invitrogen, Carlsbad, CA, USA; HIER: Heat-induced epitope retrieval; MW-microwave oven, PC-pressure cooker, S-steamer, T-trypsin and CB-citrate buffer