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. 2003 Jun 10;88(12):1925–1931. doi: 10.1038/sj.bjc.6600968

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Copyright © 2003 Cancer Research UK

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Allelic imbalance (AI). Genomic DNA was extracted from blood, tumour biopsy at diagnosis and tumour of surgical resection (patient 1). PCR of D9S171 was separated by using a sequencing analyser (ALF Pharmacia). The microsatellite was informative and shows a change in the ratio between the two amplified alleles comparing blood and paired tumour biopsies (black arrow). This patient normalised his surgical tumour sample, there is no change in the ratio between the two amplified alleles comparing blood and paired surgical tumour.