Scn1bb is expressed in the supporting cell bodies of the olfactory pits. A, Anti-Scn1bb (green) labels a ring of cell bodies in the olfactory pits (OP) and the intervening neuromasts (arrowheads) in fish that are 5 dpf. B, Anti-acetylated α-tubulin (red) labels olfactory pits in a ring corresponding to cilia and kinocilia. C, Merged image showing that anti-Scn1bb (green) and anti-acetylated α-tubulin (red) staining are nonoverlapping. D, High magnification image of a side view of an olfactory pit shows Scn1bb (green) expression in the supporting cell bodies. E, Anti-acetylated α-tubulin (red) stains the layer of kinocilia projecting from supporting cells and ciliated OSN dendritic knobs (arrows). F, Nonoverlapping expression of Scn1bb (green) in the supporting cell bodies and acetylated α-tubulin (red) in the kinocilia, cilia, and OSN dendritic knobs. Scale bars: A–C, 50 μm; D–F, 10 μm.