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. 2008 Nov 19;28(47):12510–12522. doi: 10.1523/JNEUROSCI.4329-08.2008

Figure 5.

Figure 5.

Scn1bb is expressed in the semicircular canals of the inner ear. A, Anti-Scn1bb (green) labels the epithelial pillars (ep) of the semicircular canal in 5 dpf fish. B, Anti-acetylated α-tubulin (red) labels the sensory patches found in inner ear (O, otolith; am, anterior macula; pc, posterior crista; pm, posterior macula). C, Merged image shows that expression of Scn1bb (green) and acetylated α-tubulin (red) is nonoverlapping. D, Anti-Scn1bb labels the base of the otic neuromast (arrowhead) in addition to the epithelial pillars. E, Anti-acetylated α-tubulin labels the kinocilia projecting from the otic neuromast (arrowhead). F, The two expression domains are nonoverlapping. G, Anti-Scn1bb (green) labels the otic vesicle in 72 hpf fish. H, Zn-8 (red). I, Merged image. Scale bars: A–F, 50 μm; G–I, 100 μm.