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. Author manuscript; available in PMC: 2009 Sep 10.
Published in final edited form as: Biochemistry. 2008 Jun 14;47(27):6988–6990. doi: 10.1021/bi800540b

Figure 1.

Figure 1

(a) Schematic of the experimental design. (b) A complete capture, binding, and regeneration cycle using lipoparticles on a Biacore 2000 biosensor. (c) Antibodies specific for the membrane receptors CXCR4 (12G5), CCR10 (M2 anti-FLAG), mu-opioid (M2 anti-FLAG), 5HT1a (M2 anti-FLAG), CB1 (368302), CD4 (#19), and DC-SIGN (DC11) were captured onto different flow cells of a Biacore C1 chip. Antibodies 9E10 (anti-myc), and antibodies H36 and H37 (against influenza HA) were used as negative controls. Binding was measured by flowing lipoparticles containing the specific receptor over the immobilized antibodies.