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. Author manuscript; available in PMC: 2009 Sep 10.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 May 4;293(2):L328–L335. doi: 10.1152/ajplung.00390.2006

Figure 3. Pronase Treatment.

Figure 3

A) Difference in albumin concentration (ΔN) vs. distance in a glycocalyx enzymatically digested with pronase, relative to untreated cell controls. Pronase digestion reduced ΔN at all distances above the cell surface, consistent with alteration(s) in glycocalyx structure. B) Diffusion time, τD(z), of AF532-HSA vs. distance in a glycocalyx digested with pronase, relative to solution and cell controls. Pronase digestion results in slowing of AF532-HSA diffusion at distances less than 2.0 μm above the cell membrane.