Fig. 6.

Alignment of Talpid3 proteins and domain predictions. (A) Scale and location of talpid³ mutation with highly conserved region (purple) and coiled-coil domain (green; see Fig. S4 in the supplementary material), exons 9-12 are indicated between arrows. (B) Multiple alignments of orthologous Talpid3 sequences (amino acids 295-735) from vertebrates and Nematostella vectensis using MUSCLE displayed with JALVIEW. Predicted coiled-coil region green box between amino acids 498-529; highly conserved region purple box, amino acids 498-585. Chicken sequence is used as a reference and does not contain gaps. (C) Distant sequence homologies between amino acids 392-611 (DOMAINATION domain 3) (see Table S1 in the supplementary material) of chicken Talpid3, and amino acids 0-229 of human CCCAP. Predicted coiled-coil region from PCOILS analysis (see Fig. S4 in the supplementary material) is shown by a solid outline; the highly conserved region is indicated by a broken outline. The locations of boundaries for domains encoded by exons 9-13 are shown by arrows.