Fig. 3.

Stability of HF and MF species (A) Time evolution of the 3 different DA species (HF, MF, LF) obtained by PDA for 5, 25 (e.g., Fig. S2 A and B) and 100 mM NaCl. The solid lines are fits using Eq. 18 (SI Appendix, section 3.3) with the results listed in Table S4 a and b. (B) Equilibrium fractions of HF, MF and LF (DOnly not shown) as a function of NaCl concentration. (C) Subensemble-filtered FCS curves for free DNA (black), LF+DOnly (green) and HF+MF (orange) species at 5, 25 and 100 mM NaCl. (D) Characteristic FCS diffusion times of the subspecies as a function of NaCl concentration (for all curves see Fig. S4). The data were obtained by fitting the curves with Eq. 14 (SI Appendix, section 2.5). Shaded areas indicate the 1-σ standard deviation.