Fig. 3.

Removal of Ndfip1 through shRNAi renders cells more susceptible to metal toxicity. (A) Western blot showing Ndfip1 shRNAi constructs used in (B), showing specific reduction in Ndfip1 protein compared to control with non-functional shRNAi. (B) Flow cytometry analysis of SH-SY5Y cells containing shRNAi directed toward Ndfip1 protein are more susceptible to Co toxicity compared with control cells treated with non-functional shRNAi. Two-way Anova tests (Bonferroni posttests) indicate significant cell death in Ndfip1 shRNAi cells at low concentrations of Co when compared to shRNAi control cells (P < 0.05*, ± SEM).