Fig. 7.

The E3 ligase Nedd4–2 can interact with DMT1. (A) Human primary neurons in culture for 11 days were immunoprecipitated with DMT1 antibodies and probed with antibodies from the E3 ligases Nedd4–2, or Itch. Only Nedd4–2 was observed to interact with DMT1. Cell lysates indicate that both Nedd4–2 and Itch are present in the neurons. (B) Conversely, immunoprecipitation with Nedd4–2 or Itch antibodies using SH-SY5Y cells shows a positive band for DMT1 with Nedd4–2 immunoprecipitation, but not with Itch. Reprobing demonstrated that both Nedd4–2 and Itch have been precipitated in the blots. Control lanes precipitated with protein A beads show no interactions.