Fig. 4.
Ouabain-sensitive guanidinium+-dependent currents. (A) Ouabain-sensitive currents elicited by indicated voltage pulses, from Vh = −50 mV. TEVC traces are from an oocyte injected with RDΔKESYY in 120 mM guao+ (Upper) or 120 mM Nao+ (Lower). (B) Voltage dependence of the relative amplitude (filled circles) and τ (open circles) of the slow component from biexponential fits to the traces in 120 mM guao+. (C) Same as in A but from an RD-injected oocyte. (D) Steady-state current (normalized to the 3 mM Ko+-induced current at −50 mV in NMGo+) measured from RD (filled black squares) and RDΔKESYY (open red circles, estimated from biexponential fits) expressing oocytes in 120 mM guao+.