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. Author manuscript; available in PMC: 2010 Jun 19.
Published in final edited form as: Circ Res. 2009 May 21;104(12):1355–1363. doi: 10.1161/CIRCRESAHA.108.192880

Figure 5. Lipid-loaded circulating monocytes.

Figure 5

A, LDLR-/- mice were fed a chow or high fat diet (1.25% cholesterol, 21% milk fat) for 5 weeks. Circulating mononuclear cells were isolated and stained for neutral lipid with Oil Red O.

B, Ex vivo monocytes as in A were stained with LipidTox Red to identify neutral lipid (red), Counterstaining with Alexa Fluor488 (actin cytoskeleton, green) and DAPI (nuclei, blue) demonstrates intracellular localization of the lipid. Scale, 5 μm (lipid, F-actin and merge) and 2 μm (merge-zoom).

C and D, Numbers of lipid-loaded monocytes as a function of total cholesterol and triglycerides levels in plasma.