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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Microbiol Methods. 2009 Apr 7;78(1):28–33. doi: 10.1016/j.mimet.2009.03.014

Figure 2. Bright field image of a purified protoplast fraction.

Figure 2

A. parasiticus was grown for 36 h under aflatoxin inducing conditions and protoplasts prepared and purified as described in Methods. 10μL of the pure protoplast fraction were dispensed into a hemacytometer and observed by bright field microscopy to estimate purity and total number. Size bar = 50μm.