Figure 2.

The new method for TEC isolation is reproducible and consistent. Six groups of three age‐matched thymi (18 total) were subjected to the new method for TEC isolation using greater than two technicians over multiple days. TEC, dendritic cells, endothelial cells, fibroblasts, and thymocytes were enumerated by flow cytometry after separation using the new method. The proportions of each population within the CD45 negative fraction (A) and total numbers (B) were consistent across experiments and technicians. The dendritic cell (C) and endothelial cell (D) numbers were also reliable across experiments and examiners (standard error bars shown for each population).