Fig. 2.

Survival of α β TCR KO mice following T cell transfer from vaccinated WT donor, vaccination with SIN/ZPC, and subsequent challenge with VEEV. Naïve αβ TCR KO mice were injected via i.p. route with 5 × 10⁷ T cells isolated by negative selection from spleens of vaccinated WT mice, and the purity assessed by immunostaining/flow cytometry (FACS), as described in Materials and Methods. FACS results are described in the text. As controls, mock transfer was performed in age-matched mice in parallel using PBS in identical volume. Two to five days after adoptive transfer of T cells (or mock transfer), recipient mice were immunized once (day -14, panel A), twice (day -35 and -14, panel B) or three times (day -49, -35 and -14, panel C & D) with SIN/ZPC (s.c., 5 × 10⁵ PFU per mouse). Four weeks following the final vaccination, all animals were challenged i.n. with VEEV ZPC738 (4 × 10⁵ PFU/animal). Survival curves were evaluated via logrank test (α=0.05) and the p-values are indicated on the bottom left of each graph. Asterisk (*) indicates a statistically significant result. Survival proportions of CD4⁺ or CD8⁺ transfer group was compared to the mock-transfer group via Fisher’s exact test and the p-values are indicated to the right of each curve. Double asterisk (**) indicates statistically significant results. For comparisons that were not significant (“ns”), no p-value is shown.