Fig. 2.

Age-dependent impairments of LTP but not paired-pulse facilitation in hippocampal slices from 5XFAD mice. A and B, Paired-pulse facilitation across different inter-stimulus intervals (20–500 ms) is normal in the CA1 region of 5XFAD mice at <4 months (A) and 6 months (B) of ages (n = 6–12 mice per group). C–F, LTP induced by 10-TBS (C: <4 months of age, D: 6 months of age) and 3-TBS (E: <4 months of age, F: 6 months of age) in the CA1 region of 5XFAD mice. Each point indicates the fEPSP slope normalized to the average baseline response before TBS applied at time 0. Traces are the average of fEPSPs recorded during baseline and 30–40 min after TBS. Calibration: 0.1 mV, 10 ms. G and H, The summary bar graphs show significant reductions in LTP as measured by the average normalized fEPSP slopes during 30–40 min after TBS in 5XFAD mice at 6 months of age (H) but not at <4 months of age (G) (n = 5–8 mice per group). * P<0.05 versus wild-type controls. All data are presented as mean ± SEM.