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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Cancer Res. 2009 Aug 4;69(16):6581–6589. doi: 10.1158/0008-5472.CAN-09-1161

Figure 4.

Figure 4

Up-regulation of DR4 and DR5 by zerumbone was mediated by ROS. HCT116 cells were pretreated with various concentrations of NAC (A) or GSH (B) for 1 h, then the cells were treated with 20 μmol/L zerumbone for 24 h. Whole-cell extracts were prepared and analyzed by Western blotting using DR5 and DR4 antibodies. (C) NAC reversed cell death induced by the combination of zerumbone and TRAIL. HCT116 cells were pretreated with NAC for 1 h and then treated with zerumbone for 12 h. Next, cells were washed with PBS and treated with TRAIL for 24 h. Cell death was determined by the Live/Dead Assay. (D) NAC suppressed caspase activation and PARP cleavage induced by combination of zerumbone and TRAIL. HCT116 cells were pretreated with NAC for 1 h and then treated with zerumbone for 12 h. After then, cells were washed with PBS and treated with TRAIL for 24 h. Whole-cell extracts were prepared and analyzed by Western blotting using relevant antibodies. β-actin was used as a loading control.