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Representative images of a Type II lesion inspected by CARS (A), SFG (B), and TPEF (C). (D and E) H&E staining. Wide arrow: the relative location in A–C. (F) Masson's trichrome staining. (G and H) Colocalized NLO images from the red square in A. CARS reveals aggregated lipid-laden cells (arrows) with elongated shape (possibly smooth muscle cells) in the thickened intima. (I) Colocalized image relating to the white square in H.
