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Representative images of a Type III lesion inspected by CARS (A), SFG (B), and TPEF (C). LP: lipid pools. (D) Sub-gross image of H&E staining. Wide arrow: the relative location in A–C. (E and F) Zoomed-in H&E around the pathological intima-media interface. (G) Colocalized NLO image from the red square in A shows a cell-dominated distribution with no signal of collagen. (H and I) Colocalized NLO images from the yellow square in A.
