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Representative images of a Type IV lesion inspected by CARS (A), SFG (B), and TPEF (C). The lipid core (LC) was identified by CARS. (D and E) Masson's trichrome staining. Black rectangle: the corresponding area in A–C. (F) H&E staining. (G and H) Colocalized NLO images corresponding to the red and yellow squares in A, respectively. (I) Doxorubicin-labeled (red) image, around lipid core, colocalized with CARS and SFG signals.
