Figure 5.
Representative images of a Type V lesion inspected by CARS (A), SFG (B), and TPEF (C). FC: collagen fibrous cap around the lipid core (LC). (D) Masson's trichrome stained image. Black rectangle: the correlative area in A–C. (E and F) Zoomed-in images of Masson's trichrome and H&E staining around the lipid core. Colocalized NLO image in G and doxorubicin-labeled (red) image colocalized with SFG signal in H elucidate the relationship between elongated cells (arrows) and collagenous matrix. (I) Colocalized NLO image from the yellow square in A.