Table 1.
Structure and ecoregion cluster distribution of the A. marginale MSP1a microsatellites.
| Genotype | Number of strains | m | n | SD-ATG distance (nucleotides) | Genotype frequency per ecoregion cluster | |||
| 1 | 2 | 3 | 4 | |||||
| A | 2 | 1 | 7 | 19 | 0.00 | 1.00 | 0.00 | 0.00 |
| B | 5 | 1 | 9 | 23 | 1.00 | 0.00 | 0.00 | 0.00 |
| C | 7 | 2 | 5 | 19 | 0.57 | 0.14 | 0.29 | 0.00 |
| D | 3 | 2 | 6 | 21 | 0.33 | 0.67 | 0.00 | 0.00 |
| E | 12 | 2 | 7 | 23 | 0.75 | 0.00 | 0.25 | 0.00 |
| F | 3 | 3 | 4 | 21 | 0.00 | 0.00 | 1.00 | 0.00 |
| G | 78 | 3 | 5 | 23 | 0.15 | 0.14 | 0.56 | 0.14 |
| H | 3 | 3 | 6 | 25 | 0.00 | 0.67 | 0.33 | 0.00 |
| I | NI | 4 | 6 | 29 | --- | --- | --- | --- |
The MSP1a microsatellite sequences were analyzed in 115 A. marginale strains. The microsatellite (sequence in bold) was located between the Shine-Dalgarno (SD; sequence in brackets) and the translation initiation codon (ATG) with the structure: GTAGG (G/A TTT)m (GT)n T ATG. The SD-ATG distance was calculated in nucleotides as (4 × m) + (2 × n) + 1. Abbreviation: NI, not included in the study because the A. marginale strain was not adequately geo-referenced.