Table 6.

Effect of microsatellite genotype on the expression of A. marginale MSP1a in E. coli.

A. marginale strain	Microsatellite genotype	SD-ATG distance (nucleotides)	Normalized MSP1a mRNA levels (arbitrary units)
Idaho, ID	C	19	1.57 ± 0.29 (a)
Wetumka, OK	G	23	2.31 ± 0.27 (b)
Okeechobee, FL	G	23	2.13 ± 0.0 (b)
HB-A8, China	I	29	2.39 ± 0.19 (b)

MSP1a mRNA levels were analyzed by real-time RT-PCR in three independent clones for each of the MSP1a constructs transformed in E. coli JM109. MSP1a mRNA levels were normalized against E. coli dxs gene and plasmid DNA copy number by msp1α PCR. Normalyzed MSP1a mRNA levels were represented in arbitrary units as average ± SD and compared between different constructs using an ANOVA test (different letters denote significant differences; P < 0.02).