Table 3.

PCR assay properties.

Properties	Assay 1			Assay 2
Master mix	Quantitect			Quantifast
A&E temperature	62°C			62°C
A&E time	2 min			1 min
Priming probabilities
	99% Confidence interval1			99% Confidence interval1
	Lower	Mean	Upper	Lower	Mean	Upper
T1-C2 on T-G (PM)	0.39	0.79	1.6	0.48	1.1	2.3
G1-C2 on G-G (PM)	0.49	1.0	2.1	0.50	0.95	1.8
G1-A2 on G-T (PM)	0.31	0.70	1.5	0.52	0.91	1.5
T1-C2 on G-T (2)	0.0000032	0.00021	0.0011	0.21	0.51	1.2
T1-C2 on G-G (1)	0.0090	0.015	0.025	0.41	1.2	3.5
G1-C2 on T-G (1)	0.048	0.085	0.15	0.34	0.79	1.8
G1-C2 on G-T (1)	0.0019	0.0061	0.019	0.28	0.65	1.5
G1-A2 on T-G (2)	0.00034	0.0030	0.025	0.25	0.83	2.7
G1-A2 on G-G (1)	0.0026	0.020	0.16	0.51	1.1	2.4

¹Three standard deviations above (upper) or below (lower) the mean. Means and intervals were calculated using log₂ transformed data.