Table 4.

Validation of predicted assay performance on cDNA (Molecules measured from the cDNA equivalent of 10 nanograms of total RNA from secondary phloem).

	Assay 1 (Quantitect, 2 min A&E) Primer Pairs			Assay 2 (Quantifast, 1 min A&E) Primer Pairs
Tree number (genotype)	T1-C2	G1-A2	G1-C2	T1-C2	G1-A2	G1-C2
Tree-14 (G-T/G-G)	1100	16000	18000	17000	53000	47000
Tree-17 (G-T/G-G)	1400	23000	29000	22000	54000	31000
Tree -20 (G-T/G-G)	1500	32000	21000	30000	81000	74000
Mean ± SD	1400 ± 200	24000 ± 8000	23000 ± 6000	23000 ± 7000	63000 ± 16000	51000 ± 22000
Tree-2 (G-T/G-T)	18	24000	380	11000	27000	20000
Tree-4 (G-T/G-T)	67	51000	1100	15000	35000	25000
Tree-6 (G-T/G-T)	69	54000	630	23000	60000	47000
Mean ± SD	51 ± 29	43000 ± 16000	690 ± 30	17000 ± 6000	41000 ± 17000	31000 ± 14000