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. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Aug 8;388(2):400–405. doi: 10.1016/j.bbrc.2009.08.020

Fig. 1.

Fig. 1

LRP1 3′UTR regulates target gene expression. A. The expression levels of mLRP4 without or with LRP1 3′UTR were analyzed by Western blot. Cells were co-transfected with HA-tagged mLRP4 or mLRP4 containing the 3′UTR of LRP1 and RAP. Cells were harvested 48 h later and analyzed for mLRP4 expression levels using HA antibody. B. Densitometric analysis of three independent triplicate Western blots. C. Luciferase reporter assays in cells expressing pMIR-Luc vector without or with LRP1 3′UTR. Cells were harvested 24 h later and analyzed for luciferase activity. β-gal expression vector was co-transfected for normalization of transfection efficiency. Values are the average of triple determinations with the S.D. indicated by error bars. *p < 0.05.