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. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Aug 8;388(2):400–405. doi: 10.1016/j.bbrc.2009.08.020

Fig. 2.

Fig. 2

Mir-205 down-regulates the expression of endogenous LRP1 post-transcriptionally. A. miR-205, miR-338-5p, and miR-545 seed sites within LRP1 3′UTR are shown. B. The effects of ectopic expression of miRNAs on endogenous LRP1 expression. U87 and SK-LU-1 cells were transfected with each miRNA and the endogenous expression level of LRP1 was analyzed by Western blot. Densitometric analysis of three independent Western blots is shown by bar graph. C. The expression levels of LRP1 mRNA were determined by quantitative real-time PCR following miRNA overexpression. Values are the average of triple determinations with the S.D. indicated by error bars. *p < 0.05, **p < 0.01.